Ming-Cheng Chang
Institute of Nuclear Energy Research, Taiwan
Title: Antibacterial activity of collagen/gelatin/sodium alginate biomaterial with liposomes immobilized releasing antibiotics
Biography
Biography: Ming-Cheng Chang
Abstract
Eye-drops therapy is the conventional dosage forms that account for 90% of currently accessible ophthalmic formulations. The major problem of eye-drops therapy encountered is rapid pre-corneal drug loss. Besides, high administration frequency seriously affected the quality of life of ophthalmological patients. In the current study, we developed a liposomal nanoparticle encapsulated with chloramphenicol mixed with biodegradable materials against ophthalmological disease. We first established the protocol for chloramphenicol loaded into liposomal nanoparticle; Liposomal chloramphenicol (LipoCAP). Then we established the collagen/gelatin/sodium alginate (CGA) as the component of biodegradable polymers and calibrated the novel drugs releasing formulation. Finally we combined LipoCAP with CGA to generate 8 hours-degradable ophthalmic chloramphenicol gel CGA-LipoCAP-8. Evidences indicated that CGA-LipoCAP-8 could reach the effective working concentration in 75 minutes and the drug releasing time could last for 12 hours. Besides, CGA-LipoCAP-8 could stably and continuously inhibit E. coli proliferation. The inhibiting phenomenon could be more pronounced with the time prolonging. Furthermore, there were no significant toxicities observed when CGA-LipoCAP-8 co-cultured with ocular epithelial cells. Our results of this proposal will provide innovative concepts and novel drugs releasing formulation for the future treatment of ophthalmological disease.